Genetic diversity and population structure of Rheum tanguticum (Dahuang) in China
نویسندگان
چکیده
BACKGROUND Wild Rheum tanguticum (Dahuang in Chinese) has becoming endangered in China. This study aims to examine the genetic structure and genetic diversity of R. tanguticum within species, and the genetic differentiation within and among populations in China. METHODS The variability and structure of 19 populations of R. tanguticum were investigated by their chloroplast DNA matK sequences. The genetic diversity index was calculated by Dnasp, PERMUT, and Arlequin 3.0 software, and a neighbor-joining (NJ)-tree was constructed by MEGA 5.0 software. RESULTS Fifteen haplotypes were obtained based on the matK sequence analysis. The mean genetic diversity within species was 0.894, and the genetic variability among populations (67.6%) was relatively higher than that within populations (13.88%) according to the AMOVA and PERMUT analyses. The NJ-tree and a pairwise difference analysis indicated geographical isolation of R. tanguticum. The gene flow among populations was 0.05, indicating a genetic drift among some populations, which was also confirmed by the NJ-tree and haplotype distributions. Furthermore, a mismatch distribution analysis revealed the molecular evolution of R. tanguticum. CONCLUSION Genetic diversity among and within populations of R. tanguticum in China was demonstrated.
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High Levels of Genetic Diversity within One Population of Rheum tanguti- cum on the Qinghai-Tibet Plateau have Implications for Germplasm Conservation
Rheum tanguticum, a source plant for the traditional Chinese medicine Dahuang (rhubarb), is an endangered species. In this study, we first assessed genetic diversity using 102 internal transcribed spacer (ITS) sequences cloned from one population of R. tanguticum on the Qinghai-Tibet Plateau. In total, 1773 polymorphic sites were identified, including 707 sites in the ITS1 region, 773 sites in ...
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